Research Papers

Frequency domain fluorimetry using a mercury vapor lamp

[+] Author Affiliations
Matthew J. Bohn, Michael A. Lundin, Michael A. Marciniak

Engineering Physics, Air Force Institute of Technology, 2950 Hobson Way, Wright-Patterson AFB, OH 45433

J. Appl. Remote Sens. 3(1), 033524 (April 7, 2009). doi:10.1117/1.3117448
History: Received December 8, 2008; Accepted March 19, 2009; April 7, 2009; Online April 07, 2009
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Abstract

Frequency Domain (FD) fluorimetry, capitalizes on the frequency response function of a fluorophore and offers independence from light scatter and excitation/emission intensity variations in order to extract the sample's fluorescent lifetime. Mercury vapor lamps, a common source of industrial facility lighting, emit radiation that overlaps the UV/blue absorption spectrum of many fluorophores and may be used as an efficient and portable excitation source. The AC power modulation of mercury vapor lamps modulates the lamp's intensity at 120 Hz (in the United States) and higher harmonics. The fluorescent lifetimes for 3 different materials (willemite, uranium doped glass and U3>O8>) are measured with conventional techniques and compared with the FD technique using the power harmonics from a mercury vapor lamp. The mercury lamp measurements agree to within 25% of the conventional methods.

References

National Security Program Office. Formation and Characterization of UO2F2 Particles as a Result of UF6 Hydrolysis. Oak Ridge, TN: National Security Program Office, K/NSP-777, (1998).
J. P. deNeufville, A. Kasdan, and R. J. L. Chimenti. "Selective detection of uranium by laser-induced fluorescence: a potential remote-sensing technique," Appl. Opt. 20, 1279-1296 (1981).
P. Harms, J. Sipior, N. Ram, G. Carter, and G. Rao. "Low Cost Phase-Modulation Measurements of Nanosecond Fluorescence Lifetimes Using a Lock-in Amplifier," Rev. Sci. Instrum. 70, 1535-1539 (1999).
J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd Edition. (Academic/Plenum Publishers, 1999).
M. Gaft, R. Renata, and G. Panczer. Luminescence Spectroscopy of Minerals and Materials (Springer-Verlag, 2005).
M. A. Lundin and M. J. Bohn, "Remote Sensing phase fluorimetry using a mercury vapor lamp," Proc. SPIE, 6555, (2007).
Sylvania Engineering Bulletin. SFA-HID-MEREB-069. 2006.
D. Earing, Target Signature Analysis Center: Data compilation, Second supplement. Ann Arbor, MI: University of Michigan, 8492-5-B (1967).
D. Carmer, Target Signature Analysis Center: Data compilation, Seventh supplement Ann Arbor, MI: University of Michigan, 8492-35-B (1969).
B. A. Feddersen, D. W. Piston, and E. Gratton, "Digital parallel acquisition in frequency domain fluorimetry," Rev. Sci. Instrum. 60, 2929-2936, (1989).
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Matthew J. Bohn ; Michael A. Lundin and Michael A. Marciniak
"Frequency domain fluorimetry using a mercury vapor lamp", J. Appl. Remote Sens. 3(1), 033524 (April 7, 2009). ; http://dx.doi.org/10.1117/1.3117448


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Tables

References

National Security Program Office. Formation and Characterization of UO2F2 Particles as a Result of UF6 Hydrolysis. Oak Ridge, TN: National Security Program Office, K/NSP-777, (1998).
J. P. deNeufville, A. Kasdan, and R. J. L. Chimenti. "Selective detection of uranium by laser-induced fluorescence: a potential remote-sensing technique," Appl. Opt. 20, 1279-1296 (1981).
P. Harms, J. Sipior, N. Ram, G. Carter, and G. Rao. "Low Cost Phase-Modulation Measurements of Nanosecond Fluorescence Lifetimes Using a Lock-in Amplifier," Rev. Sci. Instrum. 70, 1535-1539 (1999).
J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd Edition. (Academic/Plenum Publishers, 1999).
M. Gaft, R. Renata, and G. Panczer. Luminescence Spectroscopy of Minerals and Materials (Springer-Verlag, 2005).
M. A. Lundin and M. J. Bohn, "Remote Sensing phase fluorimetry using a mercury vapor lamp," Proc. SPIE, 6555, (2007).
Sylvania Engineering Bulletin. SFA-HID-MEREB-069. 2006.
D. Earing, Target Signature Analysis Center: Data compilation, Second supplement. Ann Arbor, MI: University of Michigan, 8492-5-B (1967).
D. Carmer, Target Signature Analysis Center: Data compilation, Seventh supplement Ann Arbor, MI: University of Michigan, 8492-35-B (1969).
B. A. Feddersen, D. W. Piston, and E. Gratton, "Digital parallel acquisition in frequency domain fluorimetry," Rev. Sci. Instrum. 60, 2929-2936, (1989).

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