Habitat loss has become one major cause of prairie loggerhead shrike population decline, which is associated with some important grassland biophysical features. However, our understanding of what and how biophysical variables can spatially characterize shrike habitats is poor. The purpose of this study is to investigate the suitability of two vegetation indices (VIs) for spatially characterizing shrike habitats in North American mixed prairies. Our research, conducted in Grasslands National Park of Canada, is based on the normalized difference vegetation index (NDVI) and the adjusted transformed soil-adjusted vegetation index (ATSAVI) as derived from both in situ measurements and SPOT imagery for three types of nesting categories at three spatial scales. Our results demonstrated that shrikes in mixed North American prairies prefer sparsely vegetated areas with a leaf area index less than 2.01 and shrub cover of around 25%. Our results also demonstrated that ATSAVI is superior to NDVI in estimating vegetation abundance and structure. Loggerhead shrikes seems to prefer habitats characterized by NDVI ranging from 0.562 to 0.616 and ATSAVI ranging from 0.319 to 0.372 with the spatial scale varying from 100 to 20 m. ATSAVI also had better performance in detecting the spatial variation of shrike habitats due to its higher sensitivity to background information.