This paper analyzes the in-flight performance of the Suomi National Polar-orbiting Partnership Ozone Mapping & Profiling Suite (OMPS) nadir instruments and evaluates sensors’ on-orbit calibrations after sensors’ two-year operation. All uncertainty values quoted in this paper are values unless stated otherwise. With the data collected from in-flight nominal calibration, our results have demonstrated that sensor performance complies with the system specifications in most cases. The largest term in the wavelength-dependent albedo calibration uncertainty for Nadir Mapper is the cross-track position difference effect of 2.5%. Final adjustments of stray light and wavelength variation are still being made to optimize OMPS sensor data records before reaching the validation mature level.