A field located on the Texas A&M University farm in Burleson County, Texas, was prepared and planted with cotton (Deltapine 1050, Monsanto, St. Louis, Missouri) at a seed spacing of 10 cm (4 in.) and row spacing of 1.02 m (40 in.). Three shred date treatments and a nonshredded control were replicated four times (blocks) in a Latin square plot design to produce various stages of regrowth cotton plants. Each plot was 48 rows by 15.24 m, and the three shred dates were 30 July, 13 August, and 27 August, respectively. These dates cover the range of dates that cotton would be harvested and shredded in South Texas. A defoliant (Thidiazuron 4 SC, Arysta Lifescience North America Corp., Cary, North Carolina) was applied at a rate of 0.224 kg ai per ha (0.2 lb ai per acre) approximately two weeks prior to shredding plants in respective plots. A Cessna 206 fixed-wing aircraft equipped with two nadir-oriented, Canon 5D Mark II digital cameras, captured 21-megapixel images defined by a array of 16-bit pixels. One camera recorded a color image (broadband red-green-blue, RGB) (ISO 200, 2-ms exposure, ), and a second camera recorded a near-infrared image (broadband NIR) (ISO 200, 2-ms exposure, ). The spectral bands were 400 to 500 nm (blue), 500 to 600 nm (green), 600 to 700 nm (red), and 720 to 1000 nm (NIR). Two spectral reflectance indices, NDVI and Green NDVI (GNDVI), were derived from the measured spectral band measurements, where and . Airborne multispectral images ( viewing area with 0.1-m pixel resolution) were acquired from flights at an altitude of above ground level. Four tarps with unique reflectance attributes (4%, 16%, 32%, and 48%) were placed near the field and within the field of view of each airborne multispectral image. The reflectance tarps were used to establish the regression fit between measured spectral reflectance (absolute digital number, DN) and relative reflectance (%) for each spectral band. Multispectral imaging flights were made on 29 June, 16 July, 26 July, 9 August, 22 August, 6 September, 20 September, 4 October and 19 October 2012 before the mandatory cotton stalk destruction date of 31 October.