Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion

Eiji Hase; Takeo Minamikawa; Shuji Miyamoto; Yasuhiro Mizutani; Tetsuo Iwata; Hirotsugu Yamamoto; Takeshi Yasui

doi:10.1117/12.2318671

24 April 2018 Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion

Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111O (2018) https://doi.org/10.1117/12.2318671
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Confocal laser microscope (CLM) has been widely used in the fields of the non-contact surface topography, biomedical imaging, and other applications, because the confocality gives two-dimensional (2D) optical-sectioning or threedimensional (3D) imaging capability and the stray light elimination. One potential method for scan-less 2D CLM is a combination of the line-focused CLM with the 1D spectral encoding CLM. In this paper, we constructed such scan-less 2D CLM with the image acquisition time of 0.23 ms, the lateral resolution of 1.2 μm, the depth resolution of 2.4 μm, and apply it for in situ imaging of plant leaf to investigate a potential for plant health monitoring.

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Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto, and Takeshi Yasui "Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111O (24 April 2018); https://doi.org/10.1117/12.2318671

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