Isotropic quantitative phase imaging with optimal differential phase contrast illumination scheme

Yao Fan; Jiasong Sun; Qian Chen; Xiangpeng Pan; Chao Zuo

doi:10.1117/12.2547850

16 October 2019 Isotropic quantitative phase imaging with optimal differential phase contrast illumination scheme

Yao Fan, Jiasong Sun, Qian Chen, Xiangpeng Pan, Chao Zuo

Proceedings Volume 11205, Seventh International Conference on Optical and Photonic Engineering (icOPEN 2019); 112050J (2019) https://doi.org/10.1117/12.2547850
Event: Seventh International Conference on Optical and Photonic Engineering (icOPEN 2019), 2019, Phuket, Thailand

Abstract

Quantitative phase imaging (QPI), which provides unique imaging capabilities for optical thickness variation of living cells and tissues without the need for specific staining or exogenous contrast agents (e.g., dyes or fluorophores), has emerged as an invaluable optical tool for biomedical research. Differential phase contrast (DPC) is the most promising QPI approach to high resolution label-free cellular dynamic imaging because of its advantages of higher imaging efficiency, higher accuracy, and higher stability. Typically, illuminations in DPC systems are designed with 2-axis half-circle amplitude patterns, which however results in a non-isotropic phase transfer function (PTF). Furthermore, the frequency responses of the PTFs have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. In this paper, we derive the optimal illumination scheme to maximize the PTF response for both low and high frequencies (from 0 to 2NA_obj), and meanwhile achieve perfectly isotropic PTF with only 2-axis intensity measurements. We present the theoretical analysis, simulations, and experimental results demonstrating that our optimal illumination scheme is a simple, efficient, and stable approach for label-free quantitative cell imaging with subcellular resolution.

Citation Download Citation

Yao Fan, Jiasong Sun, Qian Chen, Xiangpeng Pan, and Chao Zuo "Isotropic quantitative phase imaging with optimal differential phase contrast illumination scheme", Proc. SPIE 11205, Seventh International Conference on Optical and Photonic Engineering (icOPEN 2019), 112050J (16 October 2019); https://doi.org/10.1117/12.2547850

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Phase transfer function

Phase contrast

Phase imaging

Image resolution

LCDs

Biomedical optics

Microscopy

Keywords/Phrases

Search In:

Publication Years