Influence of different magnetic composites carriers on the immobilization of laccase

Haiyan Xiao; Jun Huang; Bin Li; Juntao Wang; Desheng Jiang

doi:10.1117/12.667657

2 February 2006 Influence of different magnetic composites carriers on the immobilization of laccase

Haiyan Xiao, Jun Huang, Bin Li, Juntao Wang, Desheng Jiang

Proceedings Volume 6026, ICO20: Biomedical Optics; 60261G (2006) https://doi.org/10.1117/12.667657
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

Laccase (E.C.1.10.3.2) has been used in various fields and enzyme immobilization technology is an effective means to perform enzyme reuse and to improve its stability. Carrier materials play an important role in the application of an immobilized enzyme. Magnetic carriers have been widely used in the field of protein and enzyme immobilization. The most important parameters of magnetic carriers are size, structure, density of reactive surface groups and the superparamagnetic property. The copper tetraaminophthalocyanine (CuTAPc)- Fe₃O₄ nano particle composite and chitosan-Fe₃O₄ microspheres composite were successfully synthesized and characterized by FTIR spectra, XRD and SEM micrograph. Active amino groups of two magnetic carriers could be used to bind laccase via glutaraldehyde. The optimal pH of the two immobilized laccases were the same at pH 3.0. The optimal temperature of laccase immobilized on CuTAPc-Fe₃O₄ nano particle was 45°C and that of the chitosan-Fe₃O₄ microspheres was 55°C. The immobilization yields of the two immobilized laccases were 5mg/g and 16mg/g, respectively. The Km value of the laccase immobilized on CuTAPc-Fe₃O₄ nano particles was 23.8μM, lower than that of the laccase immobilized on chitosan-Fe₃O₄ microspheres, 171.1μM. The laccase immobilized on magnetic composites could be used as biological sensing materials for biosensor.

Citation Download Citation

Haiyan Xiao, Jun Huang, Bin Li, Juntao Wang, and Desheng Jiang "Influence of different magnetic composites carriers on the immobilization of laccase", Proc. SPIE 6026, ICO20: Biomedical Optics, 60261G (2 February 2006); https://doi.org/10.1117/12.667657

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