Quantum dots photoligated with zwitterionic coating for immunofluorescence and imaging

Hedi Mattoussi; Wentao Wang

doi:10.1117/12.2610504

3 March 2022 Quantum dots photoligated with zwitterionic coating for immunofluorescence and imaging

Hedi Mattoussi, Wentao Wang

Proceedings Volume PC11977, Colloidal Nanoparticles for Biomedical Applications XVII; PC1197703 (2022) https://doi.org/10.1117/12.2610504
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We detail the design and optimization of a set of multicoordinating ligands that combine the benefits of mixed coordination (thiol and imidazole) with molecular-scale zwitterion motifs, yielding sterically stabilized and compact nanocrystals. Conjugating the resulting QDs to anti-tropomyosin receptor kinase B antibody (α-TrkB), or to the brain-derived neurotrophic factor (BDNF), has allowed successful labeling of the tropomyosin receptor kinase B (TrkB) in pyramidal neurons within cortical tissue. It has also allowed visual monitoring of BDNF-induced activation of TrkB signaling in live neuronal cells.

Conference Presentation

Citation Download Citation

Hedi Mattoussi and Wentao Wang "Quantum dots photoligated with zwitterionic coating for immunofluorescence and imaging", Proc. SPIE PC11977, Colloidal Nanoparticles for Biomedical Applications XVII, PC1197703 (3 March 2022); https://doi.org/10.1117/12.2610504

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