Resolving the sequence of DNA and RNA strands by tip-enhanced Raman spectroscopy

Jonathan Hu; Zhe He; Alexei Sokolov; Xing Wang; Marlan Scully

doi:10.1117/12.2610375

5 March 2022 Resolving the sequence of DNA and RNA strands by tip-enhanced Raman spectroscopy

Jonathan Hu, Zhe He, Alexei Sokolov, Xing Wang, Marlan Scully

Proceedings Volume PC12005, Smart Photonic and Optoelectronic Integrated Circuits 2022; PC1200503 (2022) https://doi.org/10.1117/12.2610375
Event: SPIE OPTO, 2022, San Francisco, California, United States

Abstract

Tip-enhanced Raman scattering (TERS) is a promising optical and analytical technique. TERS signals generated by a gap-mode configuration where a tip is coupled with a gold substrate can resolve DNA and RNA molecules at a single-molecule resolution. Through our deposition procedure, the molecules can be stretched, uncoiled, and attached to the substrate by its phosphate groups while exposing its nucleobases to the tip. The proof-of-principle RNA sequencing approach significantly advances a direct RNA sequencing technique without RNA labeling or amplification via reverse transcriptase RT-PCR. The demonstrated technique holds promise for next-generation DNA and RNA sequencing.

Conference Presentation

Citation Download Citation

Jonathan Hu, Zhe He, Alexei Sokolov, Xing Wang, and Marlan Scully "Resolving the sequence of DNA and RNA strands by tip-enhanced Raman spectroscopy", Proc. SPIE PC12005, Smart Photonic and Optoelectronic Integrated Circuits 2022, PC1200503 (5 March 2022); https://doi.org/10.1117/12.2610375

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