Laser induced forward transfer technique for the immobilization of biomaterials in biosensors applications (Conference Presentation)

Symeon Papazoglou; Marianeza Chatzipetrou; Maria Massaouti; Ioanna Zergioti

doi:10.1117/12.2250841

19 April 2017 Laser induced forward transfer technique for the immobilization of biomaterials in biosensors applications (Conference Presentation)

Symeon Papazoglou, Marianeza Chatzipetrou, Maria Massaouti, Ioanna Zergioti

Author Affiliations +

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 1006106 (2017) https://doi.org/10.1117/12.2250841
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Laser Induced Forward Transfer (LIFT) is a direct write technique, able to create micropatterns of biomaterials on sensing devices. In this conference we will present a new approach using LIFT for the printing and direct immobilization of biomaterials on a great variety of surfaces, for bio-sensor applications. The basic requirement for the fabrication of a biosensor is to stabilize a biomaterial that brings the physicochemical changes in close proximity to a transducer. In this direction, several immobilization methods such as covalent binding and crosslinking have been implemented. The presence of the additional functionalization steps in the biosensors fabrication, is among the main disadvantages of chemical immobilization methods. Our approach employs the LIFT technique for the direct immobilization of biomaterials, either by physical adsorption or by covalent bonding of the biomaterials. The physical adsorption of the biomaterials, occurs on hydrophobic or super-hydrophobic surfaces, due to the transition of the wetting properties of the surfaces upon the impact of the biomaterials with high velocity. The unique characteristic of LIFT technique to create high speed liquid jets, leads to the penetration of the biomaterial in the micro/nano roughness of the surface, resulting in their direct immobilization, without the need of any chemical functionalization layers. Moreover, we will also present the direct immobilization of biomaterials on Screen Printed Electrodes, for enzymatic biosensors, with a limit of detection (LOD) for catechol at 150 nM, and protein biosensors, used for the detection of herbicides, with an LOD of 8-10 nM.

Conference Presentation

Citation Download Citation

Symeon Papazoglou, Marianeza Chatzipetrou, Maria Massaouti, and Ioanna Zergioti "Laser induced forward transfer technique for the immobilization of biomaterials in biosensors applications (Conference Presentation)", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006106 (19 April 2017); https://doi.org/10.1117/12.2250841

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Biosensors

Laser applications

Adsorption

Biosensing

Electrodes

Liquids

Printing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years