Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells

Xiang-Ping Meng; Zhen Zhang; Yi-Fei Wang; Zhi-ping Wang; Tong-sheng Chen

doi:10.1117/12.2251116

20 February 2017 Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells

Xiang-Ping Meng, Zhen Zhang, Yi-Fei Wang, Zhi-ping Wang, Tong-sheng Chen

Proceedings Volume 10065, Biophotonics and Immune Responses XII; 100650U (2017) https://doi.org/10.1117/12.2251116
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Puerarin (Pue), a major active ingredient in the traditional Chinese medicine Gegen, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Pue nanosuspension (Pue-NS) composed of Pue and poloxamer 188 was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Pue-NS relative to efficacy of bulk Pue were evaluated. The particle size and zeta potential of Pue-NS were 218.5 nm and −18.8 mV, respectively. MTT assay showed that Pue-NS effectively inhibited the proliferation of HepG2 cells, and the corresponding IC50 values of Pue-NS and bulk Pue were 3.39 and 5.73 μg/ml. These results suggest that the delivery of Pue-NS is a promising approach for treating tumors.

Citation Download Citation

Xiang-Ping Meng, Zhen Zhang, Yi-Fei Wang, Zhi-ping Wang, and Tong-sheng Chen "Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells", Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650U (20 February 2017); https://doi.org/10.1117/12.2251116

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