Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy

Adam M. Hanninen; Eric O. Potma

doi:10.1117/12.2253295

2 March 2017 Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy

Adam M. Hanninen, Eric O. Potma

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100692H (2017) https://doi.org/10.1117/12.2253295
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We discuss the development and application of a laser-scanning, nonlinear optical microscope capable of generating vibrationally resonant images based on sum-frequency generation (SFG), coherent anti-Stokes Raman scattering (CARS) or third-order sum-frequency generation (TSFG). The combination of these three modalities allows vibrationally sensitive imaging of both χ ⁽²⁾ and χ ⁽³⁾-active structures in biological tissues, addressing both Raman-active as well as IR-allowed vibrational modes. We show the practical utility of these vibrationally sensitive modalities by imaging collagen I rich tissues.

Citation Download Citation

Adam M. Hanninen and Eric O. Potma "Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692H (2 March 2017); https://doi.org/10.1117/12.2253295

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