Multi-modal microscopy platform including optical tweezers and the need to perform spherical wave vectors decompositions for optical force and signal intensity calculations (Conference Presentation)

Carlos Lenz Cesar; Wendel L. Moreira; Antonio A. R. Neves; André A. de Thomaz; Diogo B. Almeida; Adriana Fontes; Hernandes F. Carvalho; Vitor B. Pelegati

doi:10.1117/12.2277331

21 September 2017 Multi-modal microscopy platform including optical tweezers and the need to perform spherical wave vectors decompositions for optical force and signal intensity calculations (Conference Presentation)

Carlos Lenz Cesar, Wendel L. Moreira, Antonio A. R. Neves, André A. de Thomaz, Diogo B. Almeida, Adriana Fontes, Hernandes F. Carvalho, Vitor B. Pelegati

Author Affiliations +

Proceedings Volume 10347, Optical Trapping and Optical Micromanipulation XIV; 1034718 (2017) https://doi.org/10.1117/12.2277331
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

We will present the multi-modal photonic platform including Optical Tweezers, linear and non linear optics techniques in a single instrument to allow parallel information gathering during single cell processes. The platform includes the following techniques: multipoint Optical Tweezers; Laser cutting; multi/single photon fluorescence, Fluorescence Lifetime Imaging (FLIM); Förster Resonant Energy Transfer (FLIM-FRET); Fluorescence Correlation Spectroscopy (FCS); Raman; Second/Third Harmonic Generation (SHG/THG); Coherent AntiStokes Raman Scattering (CARS) and cascade CARS; Near field tip-enhancement and 1 and 2 photons Photoluminescence Excitation Spectroscopy (1-2 PLE). Next, we will discuss the issue of spherical wave vectors decomposition of any optical beam in the Fourier space without any approximation solving the problem of spherical Bessel functions cancellation in both sides of the expansion. This expansion is the necessary first step to perform optical forces, as well as optical signals intensities, of scattering/absorbing particles. The limit of Rayleigh regime is easily obtained.

Conference Presentation

Citation Download Citation

Carlos Lenz Cesar, Wendel L. Moreira, Antonio A. R. Neves, André A. de Thomaz, Diogo B. Almeida, Adriana Fontes, Hernandes F. Carvalho, and Vitor B. Pelegati "Multi-modal microscopy platform including optical tweezers and the need to perform spherical wave vectors decompositions for optical force and signal intensity calculations (Conference Presentation)", Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034718 (21 September 2017); https://doi.org/10.1117/12.2277331

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