Absolute temperature measurements in optical tweezers by synchronized position and force measurement (Conference Presentation)

Anatolii V. Kashchuk; Alexander B. Stilgoe; Timo A. Nieminen; Halina H. Rubinsztein-Dunlop

doi:10.1117/12.2274659

25 September 2017 Absolute temperature measurements in optical tweezers by synchronized position and force measurement (Conference Presentation)

Anatolii V. Kashchuk, Alexander B. Stilgoe, Timo A. Nieminen, Halina H. Rubinsztein-Dunlop

Author Affiliations +

Proceedings Volume 10347, Optical Trapping and Optical Micromanipulation XIV; 103471U (2017) https://doi.org/10.1117/12.2274659
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

Precise temperature measurements in optical traps are crucial for biological objects as they are highly sensitive to the temperature. We shift the trapped particle from the equilibrium position by moving the stage and record the position and the optical force synchronously. The absolute nature of the force detection method makes it suitable for measuring optical forces in a non-linear region of the optical trap increasing the precision to 0.2K. Moreover, the proposed method does not require a precalibration of the force detector and, therefore, can be used for temperature independent calibration.

Conference Presentation

Citation Download Citation

Anatolii V. Kashchuk, Alexander B. Stilgoe, Timo A. Nieminen, and Halina H. Rubinsztein-Dunlop "Absolute temperature measurements in optical tweezers by synchronized position and force measurement (Conference Presentation)", Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103471U (25 September 2017); https://doi.org/10.1117/12.2274659

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