Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging

Xavier Intes

doi:10.1117/12.2273926

24 August 2017 Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging

Xavier Intes

Proceedings Volume 10394, Wavelets and Sparsity XVII; 1039404 (2017) https://doi.org/10.1117/12.2273926
Event: SPIE Optical Engineering + Applications, 2017, San Diego, California, United States

Abstract

An imaging platform based on time-resolved structured light and hyperspectral single-pixel detection has been developed to perform quantitative fluorescence lifetime imaging (FLI) over a large field of view (FOV) and multiple spectral bands simultaneously. We demonstrate the potential of this new imaging platform by quantitatively imaging near-infrared FRET (Förster Resonance Energy Transfer) pair, both in vitro and in vivo. The technique is well-suited for quantitative hyperspectral lifetime imaging with high-sensitivity, paving the way for many important biomedical applications.

Citation Download Citation

Xavier Intes "Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging", Proc. SPIE 10394, Wavelets and Sparsity XVII, 1039404 (24 August 2017); https://doi.org/10.1117/12.2273926

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