Comparison of calibration and standardization approaches for fluorescence guided imaging systems to benchtop fluorescence measurements in cellular systems

Maritoni Litorja; Paul DeRose

doi:10.1117/12.2301248

19 February 2018 Comparison of calibration and standardization approaches for fluorescence guided imaging systems to benchtop fluorescence measurements in cellular systems

Maritoni Litorja, Paul DeRose

Author Affiliations +

Proceedings Volume 10486, Design and Quality for Biomedical Technologies XI; 1048608 (2018) https://doi.org/10.1117/12.2301248
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Fluorescence measurements are a staple in biomedicine, from research and discovery to more recently, for fluorescenceguided imaging systems for diagnostics and surgery. Measurement validation for clinical imagers is a challenge as it is applied to many different optical systems and probe through matrices with different optical properties in a demanding field environment. In this paper we will present approaches to fluorescence calibration for a field system, in comparison to those used in laboratory instruments for cell measurements or benchtop fluorometers. We will present the common challenges and differences, and lessons from the standardization effort of laboratory fluorescence measurements. We will discuss the conceptually different pathways to measurement traceability, between counting moles of substance and measuring light.

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Maritoni Litorja and Paul DeRose "Comparison of calibration and standardization approaches for fluorescence guided imaging systems to benchtop fluorescence measurements in cellular systems ", Proc. SPIE 10486, Design and Quality for Biomedical Technologies XI, 1048608 (19 February 2018); https://doi.org/10.1117/12.2301248

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