Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy

Katsuya Sato; Oki Matsubara; Eiji Hase; Takeo Minamikawa; Takeshi Yasui

doi:10.1117/12.2316607

24 April 2018 Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy

Katsuya Sato, Oki Matsubara, Eiji Hase, Takeo Minamikawa, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107110Z (2018) https://doi.org/10.1117/12.2316607
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

The aim of this study is to provide an in situ method to non-invasively monitor osteoblastic collagen synthesis under mechanical stimulation. We applied second-harmonic-generation (SHG) microscopy to monitor the collagen fibers produced by osteoblast-like cells. To evaluate the influence of mechanical stimulation on collagen synthesis and maturation, we compared SHG images of osteoblast-produced collagen fibers with and without a cyclic stretch stimulus. Image analysis of the average SHG intensity indicated that the amount of osteoblastic collagen synthesis was significantly enhanced by the cyclic stretch. Furthermore, the maturity of the collagen fibers was not affected in the early stage of bone formation by the mechanical stimulus.

Citation Download Citation

Katsuya Sato, Oki Matsubara, Eiji Hase, Takeo Minamikawa, and Takeshi Yasui "Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110Z (24 April 2018); https://doi.org/10.1117/12.2316607

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available