STED super-resolution imaging of mitochondria in live cells

Luwei Wang; Wei Yan; Zhigang Yang; Junle Qu II

doi:10.1117/12.2500716

23 October 2018 STED super-resolution imaging of mitochondria in live cells

Luwei Wang, Wei Yan, Zhigang Yang, Junle Qu II

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108201Y (2018) https://doi.org/10.1117/12.2500716
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

A series of new fluorescent probes were developed to carry out live cell super-resolution imaging with low STED laser power or suitable STORM working conditions. And STED-FLIM imaging of microtubules labeled with ATTO647N inside HeLa cells and the mitosis process was obtained, which provides new insight into the cell structure and functions.Finally, stochastic optical reconstruction microscopy (STORM) super-resolution imaging of mitochondrial membrane in live HeLa cells was obtained by the implementation of new fluorescent probes, improved imaging system and optimized single molecule localization algorithm. This provided an important tool and strategy for studying dynamic events and complex functions in living cells.

Citation Download Citation

Luwei Wang, Wei Yan, Zhigang Yang, and Junle Qu II "STED super-resolution imaging of mitochondria in live cells", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201Y (23 October 2018); https://doi.org/10.1117/12.2500716

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