Effect of oxidative stress stimulation on intracellular PTEN trafficking in ovarian cancer by fluorescence correlation spectroscopy

Shiqing Dong; Xiaoqiong Tang; Jiao Wang; Jianling Chen; Zhengchao Wang; Hongqin Yang

doi:10.1117/12.2501147

23 October 2018 Effect of oxidative stress stimulation on intracellular PTEN trafficking in ovarian cancer by fluorescence correlation spectroscopy

Shiqing Dong, Xiaoqiong Tang, Jiao Wang, Jianling Chen, Zhengchao Wang, Hongqin Yang

Author Affiliations +

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108202R (2018) https://doi.org/10.1117/12.2501147
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

The phosphatase and tensin homolog on chromosome 10 (PTEN) is one of important tumor suppressor proteins in ovarian cancer via negatively regulating the phosphatidylinositol 3-kinase–AKT signaling pathway and controlling genomic stability. Recent studies showed the physiological function of PTEN was closely related with its subcellular compartments. But only a few technologies could quantitatively measure the concentration of PTEN at different subcellular compartments in living cells. In this study, we used fluorescence correlation spectroscopy to measure the concentrations and dynamics of EGFP-PTEN in ovarian cancer cells HO-8910. Our results showed the increasing concentration of PTEN in the cytoplasm had an opposite trends with the nucleus after the oxidative stress stimulation which was induced by H₂O₂. Furthermore, the altered diffusion of PTEN at different subcellular compartments also illustrated the PTEN was trafficked from the cytoplasm to nucleus.

Citation Download Citation

Shiqing Dong, Xiaoqiong Tang, Jiao Wang, Jianling Chen, Zhengchao Wang, and Hongqin Yang "Effect of oxidative stress stimulation on intracellular PTEN trafficking in ovarian cancer by fluorescence correlation spectroscopy", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108202R (23 October 2018); https://doi.org/10.1117/12.2501147

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