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We propose a novel technique for volumetric spectroscopy of scattering objects. The concept can be considered as a combination of optical coherence tomography (OCT) and Fourier transform spectroscopy. The 3D imaging capability is obtained with the use of coherence gating, but the detection spectrometer is based on 2D CMOS camera that allows for single-shot acquisition of spectral data corresponding to different depths in the object. The latter is possible as the beams from reference and object arms of the OCT interferometer are incident on the diffraction grating at relative angle (α) in the plane determined by the grating lines and the optical axis. In the perpendicular plane the diffraction angles are the same for both beams. The resultant sequence of spectra is subject to 2D Fourier transformation that provides the representation of the OCT signal in form of depth dependent distribution of spectrum of light scattered from sample and the envelope corresponding to the spectrum of the light coming back from particular depth in the object can be extracted and used for calculation of light extinction in the sample. The feasibility of the spectroscopic analysis using acquired 2D interferograms was proved by realization of the differential measurement of the glass cuvette filled with water in one case and with the water solution of the indocyanine green dye in the other. The proposed measurement scheme can show great potential in spectroscopic measurement of biomedical objects, in particular in vivo, due to resistance of the signal on the motion of the object.
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Szymon Tamborski, Maciej M. Bartuzel, Krystian Wrobel, Maciej Szkulmowski, "Single-shot spectral and time domain optical coherence spectroscopy (Conference Presentation)," Proc. SPIE 10887, Quantitative Phase Imaging V, 108870I (4 March 2019); https://doi.org/10.1117/12.2511314