Quantum diffraction unlimited protocol for single-photon fluorophores

Josef G. Worboys; Daniel W. Drumm; Andrew D. Greentree

doi:10.1117/12.2541217

30 December 2019 Quantum diffraction unlimited protocol for single-photon fluorophores

Josef G. Worboys, Daniel W. Drumm, Andrew D. Greentree

Proceedings Volume 11202, Biophotonics Australasia 2019; 112021N (2019) https://doi.org/10.1117/12.2541217
Event: ANZCOP, 2019, Melbourne, Australia

Abstract

Achieving higher resolution scales in optical microscopy allow a more rigorous investigation into the detailed components of cell systems. This higher resolution is typically achieved through super-resolution techniques utilizing methods inside the wave-like nature of light such as point spread function shaping and fluorophore switching. We wish to leverage both particle-like and wave-like natures of light to make a diffraction unlimited protocol. Our protocol uses the well known Hanbury Brown and Twiss (HBT) apparatus in combination with a customized second-order cross-correlation protocol. By performing least squares fitting of the HBT and intensity measurements we obtain diffraction unlimited localization for two particles of unknown relative brightness from few measurement locations. Our results show super-resolution enhancement by an order of magnitude after 5000 detection lifetimes.

Citation Download Citation

Josef G. Worboys, Daniel W. Drumm, and Andrew D. Greentree "Quantum diffraction unlimited protocol for single-photon fluorophores", Proc. SPIE 11202, Biophotonics Australasia 2019, 112021N (30 December 2019); https://doi.org/10.1117/12.2541217

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