Hyperspectral quantitative phase imaging using lens-in-lens common-path interferometer

Alexander S. Machikhin; Olga V. Polshchikova; Alina G. Vlasova; Vitold V. Pozhar

doi:10.1117/12.2526529

3 October 2019 Hyperspectral quantitative phase imaging using lens-in-lens common-path interferometer

Alexander S. Machikhin, Olga V. Polshchikova, Alina G. Vlasova, Vitold V. Pozhar

Proceedings Volume 11207, Fourth International Conference on Applications of Optics and Photonics; 112070E (2019) https://doi.org/10.1117/12.2526529
Event: IV International Conference on Applications of Optics and Photonics (AOP 2019), 2019, Lisbon, Portugal

Abstract

Quantitative phase imaging is widely used in biomedical and industrial applications for morphology and dynamics characterization of various unstained samples with nanoscale sensitivity. Registration of phase images in multiple narrow wavelength bands enables analysis of spectral properties as well as extending the dynamic range and increasing the accuracy of quantitative phase measurements. In this paper, we present a new scheme for hyperspectral quantitative phase imaging, based on acousto-optic filtration of light in lens-in-lens common-path interferometer. It may be implemented as a PC-controlled compact add-on module for light microscope, has a robust and vibration insensitive design, and allows a quantitative phase imaging of various samples. Acousto-optic filtration provides fast and arbitrary wavelength tuning within a wide range with high spectral resolution. Choosing proper parameters of the interferometer and acousto-optical filter allows adapting the proposed scheme to many microscopy applications.

Citation Download Citation

Alexander S. Machikhin, Olga V. Polshchikova, Alina G. Vlasova, and Vitold V. Pozhar "Hyperspectral quantitative phase imaging using lens-in-lens common-path interferometer", Proc. SPIE 11207, Fourth International Conference on Applications of Optics and Photonics, 112070E (3 October 2019); https://doi.org/10.1117/12.2526529

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KEYWORDS

Interferometers

Phase imaging

Microscopes

Hyperspectral imaging

Acousto-optics

Diffraction gratings

Lenses

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