Combining 3D single molecule localization strategies and sprectral demultiplexing for bioimaging (Conference Presentation)

Clément Cabriel; Nicolas Bourg; Pierre Jouchet; Guillaume Dupuis; Cyndelia Guillaume; Aurélie Baron; Marie-Ange Badet-Denisot; Boris Vauzeilles; Christophe Leterrier; Emmanuel Fort; Sandrine Leveque-Fort

doi:10.1117/12.2545210

9 March 2020 Combining 3D single molecule localization strategies and sprectral demultiplexing for bioimaging (Conference Presentation)

Clément Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Cyndelia Guillaume, Aurélie Baron, Marie-Ange Badet-Denisot, Boris Vauzeilles, Christophe Leterrier, Emmanuel Fort, Sandrine Leveque-Fort

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460I (2020) https://doi.org/10.1117/12.2545210
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

DAISY combined Supercritical Angle Fluorescence (SAF) detection in order to provide an absolute axial reference to a point spread function engineering approach . The dual view optical setup permits to introduce a strong astigmatism and decouple lateral and axial information, thus extending the axial performances without degrading the lateral precision. This technique provides 3D absolute information over a 1-µm capture range above the glass coverslip and an axial localization precision down to 15 nm with minimal loss of lateral resolution and little sensitivity to field aberrations. We will present sequential and simultaneous up to 3 color 3D imaging in cells.

Conference Presentation

Citation Download Citation

Clément Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Cyndelia Guillaume, Aurélie Baron, Marie-Ange Badet-Denisot, Boris Vauzeilles, Christophe Leterrier, Emmanuel Fort, and Sandrine Leveque-Fort "Combining 3D single molecule localization strategies and sprectral demultiplexing for bioimaging (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460I (9 March 2020); https://doi.org/10.1117/12.2545210

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