STED imaging of cytoplasmic RNA in live cells

Jianguo Zhang; Ying He; Zhenyu Gu; Dan Zhang; Pintu Das; Junle Qu; Zhigang Yang

doi:10.1117/12.2543706

21 February 2020 STED imaging of cytoplasmic RNA in live cells

Jianguo Zhang, Ying He, Zhenyu Gu, Dan Zhang, Pintu Das, Junle Qu, Zhigang Yang

Proceedings Volume 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII; 1125411 (2020) https://doi.org/10.1117/12.2543706
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

RNAs play pivotal roles in many biological processes, such as translation, gene editing etc. Since RNAs are usually smaller in size than optical diffraction limit, it is impossible for optical microscopes to get deep insight of them. With the recent emergence of super-resolution optical imaging microscopies, it becomes possible to study the ultra-fine structure of such RNA in side cells. Different kind of fluorescent probes are used for RNA labeling and imaging inside cells. However, most of them cannot be used for live-cell super-resolution imaging, due to typical laser toxicity to live cells. Herein, we developed a new type of RNA-labeled probe, which can be used for STED imaging of RNA in living cells. We believe this probe is of great significance for studying biological behaviors of RNA in living cells.

Citation Download Citation

Jianguo Zhang, Ying He, Zhenyu Gu, Dan Zhang, Pintu Das, Junle Qu, and Zhigang Yang "STED imaging of cytoplasmic RNA in live cells", Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 1125411 (21 February 2020); https://doi.org/10.1117/12.2543706

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