Non-invasive membrane potential measurements using endogenous markers

Georgi I. Petrov; Vladislav V. Yakovlev

doi:10.1117/12.2547123

6 March 2020 Non-invasive membrane potential measurements using endogenous markers

Georgi I. Petrov, Vladislav V. Yakovlev

Proceedings Volume 11256, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII; 1125606 (2020) https://doi.org/10.1117/12.2547123
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Measurements and imaging of the electric and magnetic fields inside living cells are important for understanding many biophysical interactions. In particular, membrane potential plays a critical role in cellular communication processes, regulation of active transport and generation of action potential. While most of the active research is focused on developing fluorescent labels for detecting and quantifying membrane potential, the use of endogenous markers for the electric field potential measurements would allow extended measurements of the electric field potential, which could be important both for fundamental research and clinical studies. In this report, the vibrational Stark spectroscopy is evaluated. It is demonstrated that it has sufficient sensitivity to assess physiologically significant variations of the membrane potential.

Citation Download Citation

Georgi I. Petrov and Vladislav V. Yakovlev "Non-invasive membrane potential measurements using endogenous markers", Proc. SPIE 11256, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII, 1125606 (6 March 2020); https://doi.org/10.1117/12.2547123

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