Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba; Sudheesh K. Rajput; Manoj Kumar; Xiangyu Quan; Yosuke Tamada; Yasuhiro Awatsuji; Enrique Tajahuerce

doi:10.1117/12.2559794

22 April 2020 Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji, Enrique Tajahuerce

Proceedings Volume 11402, Three-Dimensional Imaging, Visualization, and Display 2020; 114020C (2020) https://doi.org/10.1117/12.2559794
Event: SPIE Defense + Commercial Sensing, 2020, Online Only

Abstract

Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

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Citation Download Citation

Osamu Matoba, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji, and Enrique Tajahuerce "Non-interferometric 3D fluorescence imaging for bio-applications", Proc. SPIE 11402, Three-Dimensional Imaging, Visualization, and Display 2020, 114020C (22 April 2020); https://doi.org/10.1117/12.2559794

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