CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11462 > Article
Presentation
23 August 2020 Gap plasmon-based fluorescence correlation spectroscopy for anomalous molecular diffusion
Hongki Lee, Donghyun Kim, Gwiyeong Moon, Seongmin Im
Author Affiliations +
Proceedings Volume 11462, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVIII; 114621T (2020) https://doi.org/10.1117/12.2572556
Event: SPIE Nanoscience + Engineering, 2020, Online Only
Abstract
We demonstrated gold nanodimer arrays could improve the signal-to-noise ratio (SNR) of fluorescence correlation spectroscopy (FCS). In this research, we explore the feasibility of plasmon-enhanced FCS for biomolecular study using a nanodimer array whose gap size was 18 nm. Fluorescence nanobead with a diameter of 40 nm was first examined to verify if gold nanodimer arrays can enhance SNR of fluorescence and scattering intensities. We emphasize that plasmon-enhanced FCS can improve the precision for analyzing the dynamics of the particle by combining scattering characteristics of nanodimer arrays to surface plasmon resonance imaging technique. We have also observed the fluorescence enhancement and plasmon scattering in the movement of lysosome in HEK293 cells. It was found that we could measure diffusion properties such as diffusion coefficients and anomalous exponents with a low standard deviation.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Hongki Lee, Donghyun Kim, Gwiyeong Moon, and Seongmin Im "Gap plasmon-based fluorescence correlation spectroscopy for anomalous molecular diffusion", Proc. SPIE 11462, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVIII, 114621T (23 August 2020); https://doi.org/10.1117/12.2572556
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Fluorescence correlation spectroscopy
Diffusion
Luminescence
Gold
Molecular spectroscopy
Scattering
Signal to noise ratio
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top