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6 March 2021 System characterization of time-resolved mesoscopic fluorescence molecular tomography
Shan Gao, Xavier Intes
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Proceedings Volume 11634, Multimodal Biomedical Imaging XVI; 116340V (2021) https://doi.org/10.1117/12.2579098
Event: SPIE BiOS, 2021, Online Only
Abstract
We propose a novel Time-Resolved Mesoscopic Fluorescence Molecular Tomography (TR-MFMT) system based around a supercontinuum laser across visible to NIR range and a customized Single-Photon Avalanche Diode (SPAD) array. The system characterization is performed within the settings of multi-spectral fluorescence imaging. Especially, the characterization is achieved with a challenging application: NIR FRET quantification. The characterization results demonstrate the utility of narrow instrument response function systems and provide the foundation for high-resolution imaging multiplexed molecular imaging in the mesoscopic regime.
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Shan Gao and Xavier Intes "System characterization of time-resolved mesoscopic fluorescence molecular tomography", Proc. SPIE 11634, Multimodal Biomedical Imaging XVI, 116340V (6 March 2021); https://doi.org/10.1117/12.2579098
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KEYWORDS
Luminescence
Fluorescence tomography
Tomography
Fluorescence resonance energy transfer
Fluorescence lifetime imaging
Temporal resolution
Statistical analysis
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