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5 March 2021 Autofluorescence Anisotropy based investigation of cellular heterogeneity and metabolism
Jenu V. Chacko, Mark A Tsuchida, Han-Nim Lee, Erich Grotewold, Marisa S. Otegui, Kevin Eliceiri
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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480M (2021) https://doi.org/10.1117/12.2577091
Event: SPIE BiOS, 2021, Online Only
Abstract
Autofluorescence-based optical imaging methods have advanced cellular metabolism research as a technique to non-invasively measure metabolic shifts temporally and spatially. In this work, we present metabolic investigations using fluorescence anisotropy in two well-defined plant and animal models: 1) A set of flavonoid-deficient Arabidopsis thaliana mutants which shows unique fluorescence polarization properties with varying degrees of flavonoid accumulation. 2) The cancer metabolic deformities manifested in animal cells using real-time fluorescence anisotropy based metabolic readout (4fps). These studies demonstrated the potential of using fluorescence anisotropy in a systematic way to study metabolism and cellular heterogeneity.
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Jenu V. Chacko, Mark A Tsuchida, Han-Nim Lee, Erich Grotewold, Marisa S. Otegui, and Kevin Eliceiri "Autofluorescence Anisotropy based investigation of cellular heterogeneity and metabolism", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480M (5 March 2021); https://doi.org/10.1117/12.2577091
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KEYWORDS
Fluorescence anisotropy
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