Volumetric chemical imaging in vivo by a deformable mirror-based remote-focusing stimulated Raman scattering microscope

Peng Lin; Hongli Ni; Huate Li; Yuying Tan; Nicholas A. Vickers; Thomas Bifano; Ji-Xin Cheng

doi:10.1117/12.2576997

5 March 2021 Volumetric chemical imaging in vivo by a deformable mirror-based remote-focusing stimulated Raman scattering microscope

Peng Lin, Hongli Ni, Huate Li, Yuying Tan, Nicholas A. Vickers, Thomas Bifano, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116561N (2021) https://doi.org/10.1117/12.2576997
Event: SPIE BiOS, 2021, Online Only

Abstract

Stimulated Raman scattering (SRS) microscopy enables the imaging of molecular events on a human subject in vivo, such as filtration of topical drugs through the skin and intraoperative cancer detection. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.

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Citation Download Citation

Peng Lin, Hongli Ni, Huate Li, Yuying Tan, Nicholas A. Vickers, Thomas Bifano, and Ji-Xin Cheng "Volumetric chemical imaging in vivo by a deformable mirror-based remote-focusing stimulated Raman scattering microscope", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116561N (5 March 2021); https://doi.org/10.1117/12.2576997

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