Artifacts-free Fourier ptychographic microscopy using a misaligned setup

V. Bianco; B. Mandracchia; J. Bĕhal; D. Barone; P. Memmolo; P. Ferraro

doi:10.1117/12.2592550

20 June 2021 Artifacts-free Fourier ptychographic microscopy using a misaligned setup

V. Bianco, B. Mandracchia, J. Bĕhal, D. Barone, P. Memmolo, P. Ferraro

Author Affiliations +

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 1178613 (2021) https://doi.org/10.1117/12.2592550
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

Fourier Ptychographic Microscopy (FPM) probes biological samples from multiple directions and provides amplitude and quantitative phase-contrast imaging in label-free modality with large space-bandwidth product. FPM is suitable to analyze tissues in hospitals and analysis labs by unskilled users. Whenever the FPM setup is misaligned, phase artifacts can prevent a correct retrieval of the sample complex amplitude. Here we show a blind method, named Multi-Look FPM, which eliminates the unwanted artifacts and allows non-expert users skipping the recalibration process. Multi-Look FPM is proved effective in the case of neural tissue slides, cell layers, and marine microalgae with complex inner structures.

Conference Presentation

Citation Download Citation

V. Bianco, B. Mandracchia, J. Bĕhal, D. Barone, P. Memmolo, and P. Ferraro "Artifacts-free Fourier ptychographic microscopy using a misaligned setup", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 1178613 (20 June 2021); https://doi.org/10.1117/12.2592550

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