Multimodal imaging for identification and classification of circulating tumor cells

Maria Mangini; Maria Antonietta Ferrara; Gianluigi Zito; Stefano Managò; Giuseppe Coppola; Anna Chiara De Luca

doi:10.1117/12.2593504

20 June 2021 Multimodal imaging for identification and classification of circulating tumor cells

Maria Mangini, Maria Antonietta Ferrara, Gianluigi Zito, Stefano Managò, Giuseppe Coppola, Anna Chiara De Luca

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117861F (2021) https://doi.org/10.1117/12.2593504
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

Here, we present a multimodal imaging approach based on Raman spectroscopy and PSDH imaging to detect circulating tumor cells. Particularly, the ability of tumor cells to internalize glucose faster than normal cells was investigated. Deuterated-glucose was used as Raman vibrational tag since its signal is present in the “silent” zone of Raman cell spectra. Raman information were correlated with the visualization of the cell birefringence by PSDH imaging. Each technique provides different information which combination gives a molecular and morphological signature of each cell, allowing to discriminate between normal and cancer cells with a sensitivity and specificity up to 80%.

Conference Presentation

Citation Download Citation

Maria Mangini, Maria Antonietta Ferrara, Gianluigi Zito, Stefano Managò, Giuseppe Coppola, and Anna Chiara De Luca "Multimodal imaging for identification and classification of circulating tumor cells", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117861F (20 June 2021); https://doi.org/10.1117/12.2593504

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