QuanTI-FRET calibration for quantitative FRET imaging in living samples

Julien Leblanc; Alexis Coullomb; Cécile M. Bidan; Fabian Wehnekamp; Chen Qian; Don C. Lamb; Aurélie Dupont

doi:10.1117/12.2615695

7 December 2021 QuanTI-FRET calibration for quantitative FRET imaging in living samples

Julien Leblanc, Alexis Coullomb, Cécile M. Bidan, Fabian Wehnekamp, Chen Qian, Don C. Lamb, Aurélie Dupont

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119220E (2021) https://doi.org/10.1117/12.2615695
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

Quantitative FRET imaging in living cells is made possible on a simple fluorescence microscope with this new method. The single-step calibration is robust and gives absolute FRET values, independent of the instrument.

Citation Download Citation

Julien Leblanc, Alexis Coullomb, Cécile M. Bidan, Fabian Wehnekamp, Chen Qian, Don C. Lamb, and Aurélie Dupont "QuanTI-FRET calibration for quantitative FRET imaging in living samples", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119220E (7 December 2021); https://doi.org/10.1117/12.2615695

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available