Design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties

Natzem A. Lima; Travis W. Sawyer

doi:10.1117/12.2608872

4 March 2022 Design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties

Natzem A. Lima, Travis W. Sawyer

Author Affiliations +

Proceedings Volume 11944, Multiscale Imaging and Spectroscopy III; 1194407 (2022) https://doi.org/10.1117/12.2608872
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Multispectral fluorescence imaging (MSFI) is a powerful imaging modality for tissue analysis and diagnostic imaging. By illuminating with distinct wavelengths of light, intrinsic biological fluorophores and labeled markers can be measured, providing information about tissue metabolism and function. MSFI has shown promise in the scope of gastrointestinal (GI) cancers such as colon and gastric cancers. Before MSFI can be used as an endoscopic diagnostic tool there requires extensive characterization of tissue properties to identify biomarker variations that occur with the onset of disease. A robust, whole organ imaging instrument to characterize autofluorescence properties would greatly inform the development of diagnostic imaging platforms. This paper reviews the design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties. We present a detailed discussion on design considerations and demonstrate excellent performance suitable to detect tissue autofluorescence.

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Citation Download Citation

Natzem A. Lima and Travis W. Sawyer "Design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties", Proc. SPIE 11944, Multiscale Imaging and Spectroscopy III, 1194407 (4 March 2022); https://doi.org/10.1117/12.2608872

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