Extracting the reduced scattering coefficient in different optical magnifications in the blue regime: theory and experiments

Channa Shapira; Inbar Yariv; Hamootal Duadi; Dror Fixler

doi:10.1117/12.2608476

3 March 2022 Extracting the reduced scattering coefficient in different optical magnifications in the blue regime: theory and experiments

Channa Shapira, Inbar Yariv, Hamootal Duadi, Dror Fixler

Author Affiliations +

Proceedings Volume 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX; 1197605 (2022) https://doi.org/10.1117/12.2608476
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Imaging inside a turbid media is range limited. In contrast, sensing the medium’s optical properties is possible in larger depths using the iterative multi-plane optical properties extraction (IMOPE) technique. It analyzes the reemitted light phase image reconstructed from the iterative multi-plane Gerchberg-Saxton (GS) algorithm. The root mean square (RMS) of the phase yields two graphs with opposite behaviors, that cross each other in μ'_s,cp. The graphs enable the extraction of the reduced scattering coefficient, μ_s', of the measured tissue. The IMOPE was originally developed for illumination of red wavelength and for biological applications and was extended to the blue regime of the electromagnetic field, which is applicable for underwater research. In this work, we aim to extend the range of μ_s' detection by optical magnification. We use a modified diffusion theory and show how μ'_s,cp shifts with the varying magnification. The theoretical results were then tested experimentally, using agar-based phantoms with varying scatterings coefficients.

Conference Presentation

Citation Download Citation

Channa Shapira, Inbar Yariv, Hamootal Duadi, and Dror Fixler "Extracting the reduced scattering coefficient in different optical magnifications in the blue regime: theory and experiments", Proc. SPIE 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX, 1197605 (3 March 2022); https://doi.org/10.1117/12.2608476

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