The development of six-channel quantitative real-time PCR filter sets for nucleic acid detection

Ruisheng Wang; Shaopeng Ren; Xiaojun Yin; Shuaifeng Zhao; Peng Gao; Jing Ma

doi:10.1117/12.2604747

13 December 2021 The development of six-channel quantitative real-time PCR filter sets for nucleic acid detection

Ruisheng Wang, Shaopeng Ren, Xiaojun Yin, Shuaifeng Zhao, Peng Gao, Jing Ma

Proceedings Volume 12071, 10th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing and Metrology Technologies; 120710C (2021) https://doi.org/10.1117/12.2604747
Event: Tenth International Symposium on Advanced Optical Manufacturing and Testing Technologies (AOMATT 2021), 2021, Chengdu, China

Abstract

Analyzed the technical requirements of filter sets applied in 6-channel Quantitative Real-time PCR nucleic acid detection, and these filters meet the requirements were designed with double sides coating on single glass substrate, while each coating was about 200 layers. These coatings were manufactured on a plasma-assisted reactive magnetic sputtering (PARMS) machine, and the thickness error is less than 0.1%. Finally, the transmittance rates of these 6-channel real-time fluorescence PCR filters are <93%, the cut-off steepness was less than 1%, and the crosstalk was less than 1% in polychromatic fluorescence PCR.

Citation Download Citation

Ruisheng Wang, Shaopeng Ren, Xiaojun Yin, Shuaifeng Zhao, Peng Gao, and Jing Ma "The development of six-channel quantitative real-time PCR filter sets for nucleic acid detection", Proc. SPIE 12071, 10th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing and Metrology Technologies, 120710C (13 December 2021); https://doi.org/10.1117/12.2604747

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