Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities

Jannis J. Schaeper; M. Charles Liberman; Tim Salditt

doi:10.1117/12.2632792

10 November 2022 Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities

Jannis J. Schaeper, M. Charles Liberman, Tim Salditt

Proceedings Volume 12242, Developments in X-Ray Tomography XIV; 1224214 (2022) https://doi.org/10.1117/12.2632792
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

Assessing the complex three-dimensional (3D) structure of the cochlea is crucial to understand fundamental aspects of signal transduction in the inner ear and is a prerequisite for the development of novel cochlear implants (CIs). Phase-contrast x-ray tomography (XPCT) offers destruction-free 3D imaging with little sample preparation, thus preserving the delicate structure of the cochlea. In this work, we present XPCT data of small-animal and human cochleae obtained at dedicated synchrotron radiation (SR) and inhouse µ-CT setups. For µ-CT we explore heavy metal stains as radiocontrast agents and show optimized phase retrieval. With the brilliant SR we achieve high contrast for unstained whole cochleae at the cellular level.

Conference Presentation

Citation Download Citation

Jannis J. Schaeper, M. Charles Liberman, and Tim Salditt "Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities", Proc. SPIE 12242, Developments in X-Ray Tomography XIV, 1224214 (10 November 2022); https://doi.org/10.1117/12.2632792

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