Presentation + Paper
14 March 2023 Miniaturized head-mounted wide-field microscope to monitor bilateral cortical activity in mice engaged in social interaction
Jessica Lucchesi, Alessandro Scaglione, Anna Letizia Allegra Mascaro, Francesco Saverio Pavone
Author Affiliations +
Proceedings Volume 12363, Multiscale Imaging and Spectroscopy IV; 1236303 (2023) https://doi.org/10.1117/12.2649848
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract

Compromised social interactions are a clear sign of several pathological conditions such as autism, schizophrenia, and social anxiety disorder. Understanding the neural mechanisms underlying social behavior plays a key role in improving treatments.

In the past, studies of social behavior in rodents have been conducted using different behavioral paradigms but have rarely been combined with the study of brain activity, this is because the available optical imaging techniques require head-fixed conditions.

Miniaturized optical systems have recently been developed to perform calcium imaging in freely moving rodents, however, current devices have a reduced field of view limiting the brain area to be investigated.

The development of a miniaturized wide-field optical system "Miniscope" has enabled the recording of neuronal dynamics in both hemispheres in free-moving mice. The Miniscope made it possible to perform social interaction studies by simultaneously monitoring neuronal activity, opening a new frontier in the field of social neuroscience.
Conference Presentation
© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jessica Lucchesi, Alessandro Scaglione, Anna Letizia Allegra Mascaro, and Francesco Saverio Pavone "Miniaturized head-mounted wide-field microscope to monitor bilateral cortical activity in mice engaged in social interaction", Proc. SPIE 12363, Multiscale Imaging and Spectroscopy IV, 1236303 (14 March 2023); https://doi.org/10.1117/12.2649848
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KEYWORDS
Animals

Calcium

Fluorescence

Head

Microscopes

Cameras

Mental disorders

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