Functional imaging of non-human primate visual cortex using a miniaturized lensless microscope

Jimin Wu; Yuzhi Chen; Ashok Veeraraghavan; Eyal Seidemann; Jacob T. Robinson

doi:10.1117/12.2647284

14 March 2023 Functional imaging of non-human primate visual cortex using a miniaturized lensless microscope

Jimin Wu, Yuzhi Chen, Ashok Veeraraghavan, Eyal Seidemann, Jacob T. Robinson

Proceedings Volume 12365, Neural Imaging and Sensing 2023; 1236504 (2023) https://doi.org/10.1117/12.2647284
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Optical imaging technology serves as a powerful tool in neuroscience study for recording large populations of neurons in vivo. Here we present a compact lensless microscope that breaks the fundamental tradeoff in lens-based imaging systems to simultaneously achieve large field-of-view (FOV) and high-resolution imaging. Our prototype lensless microscope incorporates a “contour” phase mask with an integrated illumination system giving us improved performance for allowing us to demonstrate the first functional imaging with a lensless microscope in behaving non-human primates (NHPs). Specifically, we successfully imaged over a 16 mm² FOV on primary visual cortex of NHPs, and measured how cortical activity changes as a function of the stimulus position. The extracted position tuning information from our lensless microscope has good correspondence to the ground truth captured by a tabletop widefield microscope system.

Conference Presentation

Citation Download Citation

Jimin Wu, Yuzhi Chen, Ashok Veeraraghavan, Eyal Seidemann, and Jacob T. Robinson "Functional imaging of non-human primate visual cortex using a miniaturized lensless microscope", Proc. SPIE 12365, Neural Imaging and Sensing 2023, 1236504 (14 March 2023); https://doi.org/10.1117/12.2647284

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