Calibration-free measurement of absolute optical properties of scattering samples in a standard cuvette

Giles Blaney; Angelo Sassaroli; Cristianne Fernandez; Tapan Das; Sergio Fantini

doi:10.1117/12.2651969

7 March 2023 Calibration-free measurement of absolute optical properties of scattering samples in a standard cuvette

Giles Blaney, Angelo Sassaroli, Cristianne Fernandez, Tapan Das, Sergio Fantini

Proceedings Volume 12376, Optical Tomography and Spectroscopy of Tissue XV; 1237608 (2023) https://doi.org/10.1117/12.2651969
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Measurements of absolute optical properties of scattering samples is valuable in the field of bio-medicine and beyond. However, achieving these measurements is complicated by the need for calibration and by the large sample volumes typically needed to meet common diffusion theory models. We propose a method for calibration free absolute measurements of the absorption coefficient (µa) and reduced scattering coefficient (µ 0 s ) in a relatively small volume (the size of a standard cuvette; 45 mm × 10 mm × 10 mm). This method utilizes the previously proposed Self-Calibrating (SC) / Dual-Slope (DS) geometry by placing two light sources on one face of the cuvette and two optical detectors on the opposite face. This leads to the proposal of the Dual-Ratio of the complex Transmittance (Te) (DR{Te}), a method with the same advantages as SC / DS but with less geometric requirements and constraints. Here we confirm that measurement of DR{Te} from a cuvette may be converted to absolute optical properties. We then investigate differences between two choices of forward models for optical measurements in a cuvette, either Monte-Carlo or diffusion theory. A discrepancy between the two was found, which leads to an error of 10 % in µa and no error in µ'_s when Monte-Carlo was used to generate data and diffusion theory used to invert it. This result highlights the importance of which model is chosen for the inverse problem when this method is implemented in practice. Implementations would need to evaluate different models against ground truths to identify the optimal method for the measurement of absolute optical properties in a cuvette.

Conference Presentation

Citation Download Citation

Giles Blaney, Angelo Sassaroli, Cristianne Fernandez, Tapan Das, and Sergio Fantini "Calibration-free measurement of absolute optical properties of scattering samples in a standard cuvette", Proc. SPIE 12376, Optical Tomography and Spectroscopy of Tissue XV, 1237608 (7 March 2023); https://doi.org/10.1117/12.2651969

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