Utilizing fluorescent beads to mimic single fluorophores

Sherry Yi-Ting Feng; Luis A. Alemán-Castañeda; Rodrigo Gutiérrez-Cuevas; Isael Herrera; Thomas G. Brown; Sophie Brasselet; Miguel A. Alonso

doi:10.1117/12.2664958

16 May 2023 Utilizing fluorescent beads to mimic single fluorophores

Sherry Yi-Ting Feng, Luis A. Alemán-Castañeda, Rodrigo Gutiérrez-Cuevas, Isael Herrera, Thomas G. Brown, Sophie Brasselet, Miguel A. Alonso

Author Affiliations +

Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 1238506 (2023) https://doi.org/10.1117/12.2664958
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In this paper, numerical and semi-analytical methods are used for studying how well a fluorescent bead with a given radius in a polarizer-supplemented microscopy setup, can emulate a single fluorescent molecule.

Conference Presentation

Citation Download Citation

Sherry Yi-Ting Feng, Luis A. Alemán-Castañeda, Rodrigo Gutiérrez-Cuevas, Isael Herrera, Thomas G. Brown, Sophie Brasselet, and Miguel A. Alonso "Utilizing fluorescent beads to mimic single fluorophores", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 1238506 (16 May 2023); https://doi.org/10.1117/12.2664958

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