Light sheet fluorescence microscopy with sensor-based adaptive optics and a confocal guide star

Bingxi Liu; Yang Liu; Carly Duffy; James D. Lauderdale; Peter Kner

doi:10.1117/12.2648487

16 March 2023 Light sheet fluorescence microscopy with sensor-based adaptive optics and a confocal guide star

Bingxi Liu, Yang Liu, Carly Duffy, James D. Lauderdale, Peter Kner

Proceedings Volume 12388, Adaptive Optics and Wavefront Control for Biological Systems IX; 1238804 (2023) https://doi.org/10.1117/12.2648487
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Light sheet fluorescence microscopy is an excellent tool for imaging larger and thicker model organisms such as zebrafish larvae. Samples can be imaged with high spatial and temporal resolution over large fields of view without photodamage. But light sheet microscopy still suffers from optical aberrations due to the thickness of the samples and the high resolution. We have previously demonstrated imaging of the zebrafish central nervous system and correction of aberrations using sensorless Adaptive Optics. Sensorless AO is slow because many images are required to achieve a correction. In this work, we demonstrate measuring the wavefront using a Shack-Hartmann wavefront sensor and a confocal spot as the guide star. This approach does not require special sample preparation or an additional laser. Here we demonstrate our approach by imaging fluorescent beads and inducing wavefront errors with the deformable mirror.

Conference Presentation

Citation Download Citation

Bingxi Liu, Yang Liu, Carly Duffy, James D. Lauderdale, and Peter Kner "Light sheet fluorescence microscopy with sensor-based adaptive optics and a confocal guide star", Proc. SPIE 12388, Adaptive Optics and Wavefront Control for Biological Systems IX, 1238804 (16 March 2023); https://doi.org/10.1117/12.2648487

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