Dynamic OCT to visualize development of cotyledon vessels in sprouts

Y. Lim; S. Kojima; P. Mukherjee; I. Abd El-Sadek; S. Makita; Y. Yasuno

doi:10.1117/12.2648801

16 March 2023 Dynamic OCT to visualize development of cotyledon vessels in sprouts

Y. Lim, S. Kojima, P. Mukherjee, I. Abd El-Sadek, S. Makita, Y. Yasuno

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910O (2023) https://doi.org/10.1117/12.2648801
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The cotyledons of seven radish sprouts were measured in vivo by dynamic optical coherence tomography (OCT) for 12 days. The dynamic OCT analyzed the temporal fluctuation of the OCT signal using logarithmic intensity variance (LIV) to visualize biological and intracellular activities. The vessel structures appear in the intensity images, but they are barely visible in the early stages of development. However, the vessel structures exhibit high LIV than the cotyledon from the early stages of development, resulting in high visibility of the vessel structures in the LIV images. The length of a midrib segment was measured from the LIV images of a cotyledon. The measured length was compared to the mean LIV values calculated from the manually selected regions of interest in the midrib and the cotyledon. The length and mean LIV values of the midrib suggested the association of high LIV values with fast growth. In conclusion, dynamic OCT has the potential to study the growth of vessel structures from the early stages of development.

Conference Presentation

Citation Download Citation

Y. Lim, S. Kojima, P. Mukherjee, I. Abd El-Sadek, S. Makita, and Y. Yasuno "Dynamic OCT to visualize development of cotyledon vessels in sprouts", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910O (16 March 2023); https://doi.org/10.1117/12.2648801

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