Single-shot polarization phase shifting interferometry applied to studying ampicillin diffusion through Pseudomonas aeruginosa biofilm

Arkadiusz Kuś; Aneta Węgierek-Ciuk; Katarzyna Gałczyńska; Sławomir Wąsik; Oleg Łyżwiński; Michał Arabski; Małgorzata Kujawińska

doi:10.1117/12.2673869

11 August 2023 Single-shot polarization phase shifting interferometry applied to studying ampicillin diffusion through Pseudomonas aeruginosa biofilm

Arkadiusz Kuś, Aneta Węgierek-Ciuk, Katarzyna Gałczyńska, Sławomir Wąsik, Oleg Łyżwiński, Michał Arabski, Małgorzata Kujawińska

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 126220O (2023) https://doi.org/10.1117/12.2673869
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

In this work we present a phase-retrieval-based approach to quantifying the diffusion of drugs through biomembranes or biofilms. So far, the phenomenon was studied based on fringe orientation resulting from a refractive index gradient in the vicinity of the interface. The result is usually obtained with a Mach-Zehnder interferometer with an imaging system. This approach limits the spatial resolution of the method and does not allow observation of local changes in the diffusion. For this reason, we propose to use a single-shot phase retrieval method by utilizing a polarization-sensitive CMOS sensor to obtain four phase-shifted interferograms in a polarization-modified version of the interferometer. Finally, we demonstrate the operation of the system by quantitative analysis of ampicillin diffusion through Pseudomonas aeruginosa biofilm formed on the polyethylene terepthtalate membrane.

Conference Presentation

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Arkadiusz Kuś, Aneta Węgierek-Ciuk, Katarzyna Gałczyńska, Sławomir Wąsik, Oleg Łyżwiński, Michał Arabski, and Małgorzata Kujawińska "Single-shot polarization phase shifting interferometry applied to studying ampicillin diffusion through Pseudomonas aeruginosa biofilm", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 126220O (11 August 2023); https://doi.org/10.1117/12.2673869

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