Toxicological effect evaluation of arsenic exposure in clam Ruditapes philippinarum by using FLIM

Xiao Peng; Wei Sun; Sihan Wu; Zheng Peng; Zeyu Xiao; Yifeng Deng; Jinchuan Guo; Deliang Yu; Zhen Lu; Wei Yan; Huifeng Wu; Junle Qu

doi:10.1117/12.2683147

18 July 2023 Toxicological effect evaluation of arsenic exposure in clam Ruditapes philippinarum by using FLIM

Xiao Peng, Wei Sun, Sihan Wu, Zheng Peng, Zeyu Xiao, Yifeng Deng, Jinchuan Guo, Deliang Yu, Zhen Lu, Wei Yan, Huifeng Wu, Junle Qu

Author Affiliations +

Proceedings Volume 12745, Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023); 1274513 (2023) https://doi.org/10.1117/12.2683147
Event: Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023), 2023, Haikou, China

Abstract

Inorganic arsenic (iAs) is one of the most toxic metalloids which could accumulate in marine species, especially in clams, causing serious ecological risk. Marine clams accumulate high level of iAs in different tissues. Currently, Fluorescence Lifetime Microscopy (FLIM) technique has provided quantitative information in biochemical diagnosis. In this study, we applied FLIM method into analyzing Hematoxylin and eosin (H&E) stained sections of arsenic exposed Ruditapes philippinarum. The clams were exposed under different concentrations of As(Ⅲ) and As(V) for thirty days. Fluorescent images of the H&E stained hepatopancreas tissue were obtained with FLIM system, followed with data analysis for fluorescence lifetime values. The average fluorescence lifetime of sections in the control group was around 250 ps. The average lifetime value in the As(V) group was slightly increased to around 280-300 ps. The average lifetime value in the As(III) group achieved a significant increase to around 340 ps. These results suggested a higher extent of structural change in As(Ⅲ) exposed group than As(V) group. As a result, this work has provided quantitative evaluation standard for the toxicity of marine mollusk based on fluorescence lifetime imaging method.

Citation Download Citation

Xiao Peng, Wei Sun, Sihan Wu, Zheng Peng, Zeyu Xiao, Yifeng Deng, Jinchuan Guo, Deliang Yu, Zhen Lu, Wei Yan, Huifeng Wu, and Junle Qu "Toxicological effect evaluation of arsenic exposure in clam Ruditapes philippinarum by using FLIM", Proc. SPIE 12745, Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023), 1274513 (18 July 2023); https://doi.org/10.1117/12.2683147

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Fluorescence

Fluorescence lifetime imaging

Arsenic

Tissues

Oceanography

Environmental monitoring

Pollution

Show All Keywords

Keywords/Phrases

Search In:

Publication Years