A rapid spectroscopic platform in identifying cisplatin resistance in head and neck squamous cell carcinoma

Shu-Jen Chiang; Po-Hang Tseng; Mei-Chun Lin; Chih-Yu Leu; Madhusudan Roy; Chen-Yuan Dong; Pei-Jen Lou

doi:10.1117/12.2691213

12 March 2024 A rapid spectroscopic platform in identifying cisplatin resistance in head and neck squamous cell carcinoma

Shu-Jen Chiang, Po-Hang Tseng, Mei-Chun Lin, Chih-Yu Leu, Madhusudan Roy, Chen-Yuan Dong, Pei-Jen Lou

Author Affiliations +

Proceedings Volume 12818, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2024; 1281804 (2024) https://doi.org/10.1117/12.2691213
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

At present, the administration of chemotherapy drugs is largely based on physician experience and clinical guidelines. Clearly, a personalized solution would significantly benefit patients in the improvement of clinical outcomes. In this work, we used a spectroscopic approach in which a water-soluble tetrazolium salt (WST-8) assay was applied to patient-derived slice organoids. WST-8 would react with intracellular dehydrogenase resulting in the formation of formazan dye. The absorption at 450nm can be used to assess the viability of the organoids 5 days after initiation of organoid culture. With additional development, this approach may be used to improve the clinical outcome of patients undergoing chemotherapy.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Shu-Jen Chiang, Po-Hang Tseng, Mei-Chun Lin, Chih-Yu Leu, Madhusudan Roy, Chen-Yuan Dong, and Pei-Jen Lou "A rapid spectroscopic platform in identifying cisplatin resistance in head and neck squamous cell carcinoma", Proc. SPIE 12818, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2024, 1281804 (12 March 2024); https://doi.org/10.1117/12.2691213

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