Evaluate time-course pharmacodynamics in tumor organoid with stress-induced lipofuscin-like autofluorescence

Yinghan Yan; Tzu-Ming Liu

doi:10.1117/12.3008730

12 March 2024 Evaluate time-course pharmacodynamics in tumor organoid with stress-induced lipofuscin-like autofluorescence

Yinghan Yan, Tzu-Ming Liu

Author Affiliations +

Proceedings Volume 12821, Visualizing and Quantifying Drug Distribution in Tissue VIII; 1282106 (2024) https://doi.org/10.1117/12.3008730
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Traditional drug sensitivity assay confirms the cell death by time-consuming fixation and labeling. This snapshot evaluation overlooks the valuable time-course pharmacodynamics that could offer new insights for accurate drug screening. In this study, we have developed a label-free method to promptly detect cell senescence using endogenous lipofuscin-like autofluorescence. Following drug treatment, we observed that damaged mitochondria release molecules emitting lipofuscin-like red autofluorescence. This corresponding fluorescence intensity significantly increases in apoptotic and necrotic cells. Such innovative approach enables the real-time observation of treatment outcomes in 3D tumor organoids and has the potential to determine drug sensitivity earlier than the Annexin V/PI assay. This metabolic fluorescence signature could substantially enhance the efficiency of drug sensitivity testing.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yinghan Yan and Tzu-Ming Liu "Evaluate time-course pharmacodynamics in tumor organoid with stress-induced lipofuscin-like autofluorescence", Proc. SPIE 12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, 1282106 (12 March 2024); https://doi.org/10.1117/12.3008730

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