Multi-class instance segmentation of renal structures in highly multiplexed immunofluorescence microscopy images

Thao Cao; Madeleine S. Durkee; Junting Ai; Gabriel Casella; Deepjyoti Ghosh; Anthony Chang; Michael Andrade; Maryellen L. Giger; Marcus R. Clark

doi:10.1117/12.3008544

3 April 2024 Multi-class instance segmentation of renal structures in highly multiplexed immunofluorescence microscopy images

Thao Cao, Madeleine S. Durkee, Junting Ai, Gabriel Casella, Deepjyoti Ghosh, Anthony Chang, Michael Andrade, Maryellen L. Giger, Marcus R. Clark

Author Affiliations +

Proceedings Volume 12933, Medical Imaging 2024: Digital and Computational Pathology; 129331I (2024) https://doi.org/10.1117/12.3008544
Event: SPIE Medical Imaging, 2024, San Diego, California, United States

Conference Poster

Abstract

Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus, with up to 30% of LN patients progressing to end-stage kidney disease within ten years of diagnosis. Spatial relationships between specific types of immune cells and kidney structures hold valuable information clinically and biologically. Thus, we develop a modular computational pipeline to analyze the spatially resolved molecular features from high-plex immunofluorescence imaging data. Here, we present three modules of the pipeline, with the goal of achieving multiclass segmentation of renal cells and structures.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Thao Cao, Madeleine S. Durkee, Junting Ai, Gabriel Casella, Deepjyoti Ghosh, Anthony Chang, Michael Andrade, Maryellen L. Giger, and Marcus R. Clark "Multi-class instance segmentation of renal structures in highly multiplexed immunofluorescence microscopy images", Proc. SPIE 12933, Medical Imaging 2024: Digital and Computational Pathology, 129331I (3 April 2024); https://doi.org/10.1117/12.3008544

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