Effect of mouse skin autofluorescence on the measurement of photosensitizer fluorescence

Xingran Guo; Weijin Peng; Zhe Wei; Shengdong Cui; Huiqi Zhan; Cheng Ke; Zheng Huang

doi:10.1117/12.3036356

20 November 2024 Effect of mouse skin autofluorescence on the measurement of photosensitizer fluorescence

Xingran Guo, Weijin Peng, Zhe Wei, Shengdong Cui, Huiqi Zhan, Cheng Ke, Zheng Huang

Proceedings Volume 13242, Optics in Health Care and Biomedical Optics XIV; 1324226 (2024) https://doi.org/10.1117/12.3036356
Event: SPIE/COS Photonics Asia, 2024, Nantong, Jiangsu, China

Conference Poster

Abstract

Background: Mouse model is a unique tool for preclinical evaluation of photosensitizing drugs for their potential applications in photodynamic therapy (PDT). The fluorescent property of photosensitizer provides a useful means for in situ measurement of photosensitizer distribution. However, the autofluorescence of mouse tissues might potentially affect in situ photosensitizer fluorescence measurement. Objectives: The aim of this study was to evaluate the autofluorescence in lab mice and its influence on the in situ measurement of photosensitizer fluorescence. Materials and Materials and methods: ICR mice, commonly used lab animal model, were used. Mice were fed with regular diet and clean water. Domestically produced photosensitizing drug Hemoporfin® was used as a model sensitizer. Costume-built fluorescence imaging and spectroscope systems were used for fluorescence examination under the excitation wavelength of 400 ± 5 nm, generated from a laser or LED panel. In situ fluorescence was examined before and after i.v. injection of Hemoporfin®. Results: Epifluorescence imaging examination showed yellowish and reddish autofluorescence fluorescence in the mouth, nose, paws and tail areas. The absorption and emission spectra of mouse tissue overlaid to some degrees with that of Hemoporfin®. In situ fluorescence examination of ICR mice showed significant influence of autofluorescence on the spectral and intensity measurement of Hemoporfin® fluorescence. Such influence might be eliminated by post-measurement spectral correction algorithms. Conclusions: The autofluorescence in mouse tissues poses a significant influence on the in situ measurement of fluorescence emission of Hemoporfin®. Careful postmeasurement spectral correction is needed for accurately measuring photosensitizer fluorescence.

Citation Download Citation

Xingran Guo, Weijin Peng, Zhe Wei, Shengdong Cui, Huiqi Zhan, Cheng Ke, and Zheng Huang "Effect of mouse skin autofluorescence on the measurement of photosensitizer fluorescence", Proc. SPIE 13242, Optics in Health Care and Biomedical Optics XIV, 1324226 (20 November 2024); https://doi.org/10.1117/12.3036356

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available