Sequence dependence of the length of the B to Z junctions in DNA

Warner L. Peticolas; Zhongy Dai; G. A. Thomas

doi:10.1117/12.57319

1 May 1991 Sequence dependence of the length of the B to Z junctions in DNA

Warner L. Peticolas, Zhongy Dai, G. A. Thomas

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57319
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

The Raman spectra of several duplexes of self-complementary oligodeoxynucleotides such as d[TTCGCGCGCGAA] have been taken under various conditions of salt concentration and temperature. It is shown how to determine the number of CG base pairs in the Z form and how many in the junction between the B and Z forms in these oligomers. The intensities of the guanine-backbone bands at 680 cm'' and 625 cm'' are used. As noted by M. Tsuboi Y. Nishimura G. J. Thomas Jr. and their coworkers the former frequency is the marker band for the B form or the disordered form while the latter frequency is the marker band for the Z form. The marker bands of the AT base pairs previously determined by Taillandier and Liquier can be used to rule out the occurrence of the Z form in the AT portion of the duplex. From these measurements the structure of the double helical duplex in concentrated salt solutions can be described as having four of the six interior CG base pairs in Z form flanked by two CG base pairs in the junction and the two AT base pairs in a short B form segment. Similar measurements on a large number of oligomers shows that both single B-Z and double B-Z-B junctions may be present both in the solution and in single crystals.

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Warner L. Peticolas, Zhongy Dai, and G. A. Thomas "Sequence dependence of the length of the B to Z junctions in DNA", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57319

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KEYWORDS

Raman spectroscopy

Laser applications

Crystals

Argon ion lasers

Error analysis

Life sciences

Chemistry

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